![]() The Euroline WB has a consistently high sensitivity, but specificity varied significantly among the different locations. Among the 437 Ugandans in Rakai, 67.3% were HSV-2-positive by Euroline WB (sensitivity 98.7%, specificity 65.4%). Fluorescent Western Blotting Gel Drying Equipment In-Cell Western Assay Preparative Electrophoresis Protein Gels Protein Stains Protein Ladders and. Among the 273 commercial sex workers in Kunming, 62.3% were HSV-2-positive by Euroline WB (sensitivity 96.9%, specificity 89.1%). Among the 135 American men attending a STI clinic in Baltimore, MD, 72% (n = 97) were HSV-2-positive by Euroline WB, showing a sensitivity of 97.8% and a specificity of 81.8%. Herpes Simplex Virus Type 2 (HSV2) Western Blot Confirmatory Testing Among Men Testing Positive for HSV2 Using the Focus Enzyme-Linked Immunosorbent Assay in a. This study evaluated Euroline WB for the detection of HSV-2 antibodies compared with University of Washington Western blot in three geographically different regions: Baltimore, MD, USA Rakai, Uganda and Kunming, China. A study in which herpes simplex virus type 2 (HSV-2) Western blots were performed on patients testing positive for HSV-2 using the Focus HSV-2 enzyme-linked immunosorbent assay (ELISA) suggests that a higher ELISA index value cutoff should be used when testing persons with antibody to herpes simplex virus type 1 or persons without a history of clinical evidence of genital herpes. The sensitivity and specificity were determined with samples from ten sites (n 967. A subset of sera from each site was then selected, based on the HSV-2 results, and tested by Western blot (WB). ![]() HSV-2 is the main cause of genital ulcers and is diagnosed mainly with serological tests. Performance of focus ELISA tests for herpes simplex virus type 1 (HSV-1) and HSV-2 antibodies among women in ten diverse geographical locations. The Euroline Western blot (WB) is a commercially available assay that is easy to perform however, little is known about its performance characteristics. Background and aims: Herpes simplex virus type 2 (HSV-2) is a sexually transmitted agent and is detected worldwide. While glycoprotein G-2 enzyme-linked immunosorbent assays are commonly used for the serological detection of HSV-2 antibodies, they have low specificity in developing countries. Sensitivity ranged from 93 to 96 and specificity from 95 to 98 13, 25, 26. Herpes simplex virus type 2 (HSV-2) infection is one of the most common sexually transmitted infections (STIs) worldwide. The POCkit-HSV-2 test has been tested against the CPHL monoclonal antibody blocking ELISA and against the University of Washington Western blot. The Herpes Western Blot has a very high rate of accuracy one month after becoming infected with HSV, all though it can sometimes be detected in a shorter amount.
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